Selected liquid protein fractions can be further analyzed by mass spectrometry. The system has an automated injection module for sample handling between first and second dimension columns, a sensitive UV detector and an automated 96-well format robot for collection of samples from the second dimension column. ProteomeLab PF2D (Beckman Coulter) is a high resolution 2-dimensional liquid protein fractionation system using chromatofocusing in the first dimension and a high capacity reverse phase HPLC column in the second phase. Two dimensional liquid chromatography for patient serum/plasma samples, solid tumors, and cultured cells. Protein Profiling for Biomarker Discovery and Differential Expression Many others (please inquire or check Luminex website for more information).Cytokines, adipokines, & growth factors.Assays can be done with small samples of cell and tissue extracts, cell culture media, serum, and other biologic fluids. Beads coupled with different antibodies, each with a distinct fluorescence signature, are mixed, thus enabling simultaneous assay of multiple protein targets in a single well of a 96-well plate. The beads with bound target proteins are separated by laser excitation and quantitated. In the Luminex assays, a specific antibody to the target protein is covalently coupled to internal fluorescently dyed beads. Multiplex assays provide attractive time and labor advantages over singleplex ELISA-based assays.
Luminex’s xMAP technology is a microsphere-based multiplexing system. Multiplex quantitative analyses using the suspension array system (Bio-plex from Bio-Rad) through Luminex xMAP bead technology
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